4/11/2023 0 Comments Ecd detector liner![]() Column bleed needs to be discriminated from other similar bleedings such as septum bleed, column contamination and fouling of detector or liner. The region is generally free of any distinct peaks. Comparison between bleeding and normal column chromatogramsĬolumn bleeding is characterized by a steady rise in the baseline about 30 degrees C before the upper permissible temperature limit and reaches a plateau around the upper limit. It is natural for all columns, irrespective of stationary phase film thickness, to bleed at high temperatures but excessive bleeding results in shortening of the useful lifespan of the columns. Thick films columns are used to handle higher sample loads but at same time their bleed rates are higher. The level of bleed depends on the film thickness of the stationary phase layer on the adsorbent. ![]() Column bleeding results from thermal breakdown of the stationary phase at temperatures close to the upper temperature limit of the column. ![]() Reduction of column bleed requires first of all a clear understanding of the term.
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